2,270 research outputs found
Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila.: oocyte fate and meiosis control
Meiosis is a specialized cell cycle limited to the gametes in Metazoa. In Drosophila, oocyte determination and meiosis control are interdependent processes, and BicD appears to play a key role in both. However, the exact mechanism of how BicD-dependent polarized transport could influence meiosis and vice versa remains an open question. In this article, we report that the cell cycle regulatory kinase Polo binds to BicD protein during oogenesis. Polo is expressed in all cells during cyst formation before specifically localizing to the oocyte. This is the earliest known example of asymmetric localization of a cell-cycle regulator in this process. This localization is dependent on BicD and the Dynein complex. Loss- and gain-of-function experiments showed that Polo has two independent functions. On the one hand, it acts as a trigger for meiosis. On the other hand, it is independently required, in a cell-autonomous manner, for the activation of BicD-dependent transport. Moreover, we show that Polo overexpression can rescue a hypomorphic mutation of BicD by restoring its localization and its function, suggesting that the requirement for Polo in polarized transport acts through regulation of BicD. Taken together, our data indicate the existence of a positive feedback loop between BicD and Polo, and we propose that this loop represents a functional link between oocyte specification and the control of meiosis
Optical Coherence Spectro-Tomography by all-Optical Depth-Wavelength analysis
Current spectroscopic optical coherence tomography (OCT) methods rely on a
posteriori numerical calculation. We present an alternative for accessing
optically the spectroscopic information in OCT, i.e. without any
post-processing, by using a grating based correlation and a wavelength
demultiplexing system. Conventional A-scan and spectrally resolved A-scan are
directly recorded on the image sensor. Furthermore, due to the grating based
system, no correlation scan is necessary. In the frame of this paper we present
the principle of the system as well as first experimental results
Observation of geometric parametric instability induced by the periodic spatial self-imaging of multimode waves
Spatio-temporal mode coupling in highly multimode physical systems permits
new routes for exploring complex instabilities and forming coherent wave
structures. We present here the first experimental demonstration of multiple
geometric parametric instability sidebands, generated in the frequency domain
through resonant space-time coupling, owing to the natural periodic spatial
self-imaging of a multimode quasi-continuous-wave beam in a standard
graded-index multimode fiber. The input beam was launched in the fiber by means
of an amplified microchip laser emitting sub-nanosecond pulses at 1064 nm. The
experimentally observed frequency spacing among sidebands agrees well with
analytical predictions and numerical simulations. The first order peaks are
located at the considerably large detuning of 123.5 THz from the pump. These
results open the remarkable possibility to convert a near-infrared laser
directly into a broad spectral range spanning visible and infrared wavelengths,
by means of a single resonant parametric nonlinear effect occurring in the
normal dispersion regime. As a further evidence of our strong space-time
coupling regime, we observed the striking effect that all of the different
sideband peaks were carried by a well-defined and stable bell-shaped spatial
profile.Comment: 5 pages, 4 figure
Nonlinear beam self-imaging and self-focusing dynamics in a GRIN multimode optical fiber: theory and experiments
Beam self-imaging in nonlinear graded-index multimode optical fibers is of
interest for many applications, such as implementing a fast saturable absorber
mechanism in fiber lasers via multimode interference. We obtain an exact
solution for the nonlinear evolution of first and second order moments of a
laser beam carried by a graded-index multimode fiber, predicting that the
spatial self-imaging period does not vary with power. Whereas the amplitude of
the oscillation of the beam width is power-dependent. We have experimentally
studied the longitudinal evolution of beam self-imaging by means of femtosecond
laser pulse propagation in both the anomalous and the normal dispersion regime
of a standard telecom graded-index multimode optical fiber. Light scattering
out of the fiber core via visible fluorescence emission and harmonic wave
generation permits us to directly confirm that the self-imaging period is
invariant with power. Spatial shift and splitting of the self-imaging process
under the action of self-focusing are also emphasized
Stable mode-locked operation of a low repetition rate diode-pumped Nd : GdVO4 laser by combining quadratic polarisation switching and a semiconductor saturable absorber mirror
International audienceIn this paper, we present the mode-locked operation of an ultrarobustly stabilised Nd:GdVO4 laser with low repetition rate by combining quadratic polarisation switching and a semiconductor saturable absorber mirror (SESAM). In addition, similar experiment was also done with Nd:YVO4. For Nd:GdVO4, 16-ps pulses at 1063nm with a repetition rate of 3.95MHz have been obtained for a laser average output power of 1.4W. For Nd:YVO4, the performance was 2.5W of average power for15-ps pulses at 1064nm. Moreover, we demonstrate experimentally the advantage of combining these two passive mode locking techniques in terms of stability ranges. We show how the dual mode-locking technique is crucial to obtain a stable and long-term mode-locked regime in our case of a diode-pumped Nd:GdVO4 laser operating at low repetition rate and more generally how this dual mode-locking technique improves the stability range of the modelocked operation giving more flexibility on different parameter
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Nonlinear polarization dynamics of Kerr beam self-cleaning in a GRIN multimode optical fiber
We experimentally study polarization dynamics of Kerr beam self-cleaning in a
graded-index multimode optical fiber. We show that spatial beam cleaning is
accompanied by nonlinear polarization rotation, and a substantial increase of
the degree of linear polarization.Comment: 5 pages, 6 figure
Nonlinear optics in multimode fibers
We overview the emerging field of nonlinear optics in multimode optical fibers, which enable new methods for the ultrafast
light-activated control of temporal, spatial and spectral degrees of freedom of intense pulsed light beams
Nonlinear dynamics of spatio-temporal waves in multimode fibres
Nonlinear multimode fibers provide an intriguing test-bed for exploring complex
spatio-temporal beam dynamics. We overview recent experimental observations of Kerr beam
self-cleaning, parametric sideband series and supercontinuum generation in passive and active
multimode optical fibers
Efficiency of dispersive wave generation in dual concentric core microstructured fiber
We describe the generation of powerful dispersive waves that are observed
when pumping a dual concentric core microstructured fiber by means of a
sub-nanosecond laser emitting at the wavelength of~1064 nm. The presence of
three zeros in the dispersion curve, their spectral separation from the pump
wavelength, and the complex dynamics of solitons originated by the pump pulse
break-up, all contribute to boost the amplitude of the dispersive wave on the
long-wavelength side of the pump. The measured conversion efficiency towards
the dispersive wave at 1548 nm is as high as 50%. Our experimental analysis of
the output spectra is completed by the acquisition of the time delays of the
different spectral components. Numerical simulations and an analytical
perturbative analysis identify the central wavelength of the red-shifted pump
solitons and the dispersion profile of the fiber as the key parameters for
determining the efficiency of the dispersive wave generation process.Comment: 11 pages, 12 figure
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